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Md Shivli Nomani1, Jeyabalan Govinda Samy2

1Department of Pharmacy, SunRise University, Bagard Rajput, Alwar, Rajasthan, India.
2Department of Pharmacy, Alwar Pharmacy College, Alwar, Rajasthan, India.

Volume 3, Issue 3, Page 122-131, September-December 2015.

Article history
Received: 15 December 2015
Revised: 25 December 2015
Accepted: 27 December 2015
Early view: 29 December 2015

*Author for correspondence

Objective: The objective of the present study was to formulate, optimize and characterize dual drug loaded nanoliposomal system containing metformin hydrochloride (MTN) and glimepiride (GLD).
Material and methods: Nanoliposomal formulations were optimized by Box-Behnken design using ethanol injection method. A 3-factor, 3-levels Box-Behnken design was used to derive a second order polynomial equation and construct contour plots to predict responses. The independent variables selected were molar ratio phospholipid:cholesterol (X1), aqueous phase concentration (X2) and lipid phase concentration (X3). The responses analyzed were particle size and entrapment efficiency.
Results: The final optimized formulation (LP-F) had the particle size, polydispersity index (PDI), %EEMTN, %EEGLD and %yield of 180.3 ± 2.69nm, 0.13 ± 0.07, 88.6 ± 1.99%, 99.3 ± 0.13% and 95.6 ± 1.23% respectively. TEM revealed Vesicles were dispersed and non-aggregated form with the average size of 185 ± 4.76 nm. SEM revealed 3D- surface view of the liposome which exposed spherical uniformly distributed particles.
Conclusion: From in-vitro release profiles of LP-F, it was observed that both the drugs have nearly simultaneous release profile in sustained manner.
Keywords: Nanoliposome, Box-Behnken dsign, Ethanol injection method, Antidiabetic, Metformin hydrochloride, Glimepride.