Md Shivli Nomani1, Jeyabalan Govinda Samy2
1Department of Pharmacy, SunRise University, Bagard Rajput, Alwar, Rajasthan, India.
2Department of Pharmacy, Alwar Pharmacy College, Alwar, Rajasthan, India.
ORIGINAL RESEARCH ARTICLE
Volume 3, Issue 3, Page 122-131, September-December 2015.
Article history
Received: 15 December 2015
Revised: 25 December 2015
Accepted: 27 December 2015
Early view: 29 December 2015
*Author for correspondence
E-mail: [email protected]
ABSTRACT
Objective: The objective of the present study was to formulate, optimize and characterize dual drug loaded nanoliposomal system containing metformin hydrochloride (MTN) and glimepiride (GLD).
Material and methods: Nanoliposomal formulations were optimized by Box-Behnken design using ethanol injection method. A 3-factor, 3-levels Box-Behnken design was used to derive a second order polynomial equation and construct contour plots to predict responses. The independent variables selected were molar ratio phospholipid:cholesterol (X1), aqueous phase concentration (X2) and lipid phase concentration (X3). The responses analyzed were particle size and entrapment efficiency.
Results: The final optimized formulation (LP-F) had the particle size, polydispersity index (PDI), %EEMTN, %EEGLD and %yield of 180.3 ± 2.69nm, 0.13 ± 0.07, 88.6 ± 1.99%, 99.3 ± 0.13% and 95.6 ± 1.23% respectively. TEM revealed Vesicles were dispersed and non-aggregated form with the average size of 185 ± 4.76 nm. SEM revealed 3D- surface view of the liposome which exposed spherical uniformly distributed particles.
Conclusion: From in-vitro release profiles of LP-F, it was observed that both the drugs have nearly simultaneous release profile in sustained manner.
Keywords: Nanoliposome, Box-Behnken dsign, Ethanol injection method, Antidiabetic, Metformin hydrochloride, Glimepride.
Objective: The objective of the present study was to formulate, optimize and characterize dual drug loaded nanoliposomal system containing metformin hydrochloride (MTN) and glimepiride (GLD).
Material and methods: Nanoliposomal formulations were optimized by Box-Behnken design using ethanol injection method. A 3-factor, 3-levels Box-Behnken design was used to derive a second order polynomial equation and construct contour plots to predict responses. The independent variables selected were molar ratio phospholipid:cholesterol (X1), aqueous phase concentration (X2) and lipid phase concentration (X3). The responses analyzed were particle size and entrapment efficiency.
Results: The final optimized formulation (LP-F) had the particle size, polydispersity index (PDI), %EEMTN, %EEGLD and %yield of 180.3 ± 2.69nm, 0.13 ± 0.07, 88.6 ± 1.99%, 99.3 ± 0.13% and 95.6 ± 1.23% respectively. TEM revealed Vesicles were dispersed and non-aggregated form with the average size of 185 ± 4.76 nm. SEM revealed 3D- surface view of the liposome which exposed spherical uniformly distributed particles.
Conclusion: From in-vitro release profiles of LP-F, it was observed that both the drugs have nearly simultaneous release profile in sustained manner.
Keywords: Nanoliposome, Box-Behnken dsign, Ethanol injection method, Antidiabetic, Metformin hydrochloride, Glimepride.
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