Background: : Doxorubicin (DXN) is the most active cytotoxic agents having efficacy in malignancies either alone or combined with other cytocidal agents. The clinical usefulness of the anthracycline drug has been precluded by oxidative stress. This study is based on the possible protective effect of hydroalcoholic extract of Vitis vinifera against DXN-induced oxidative stress in male Wistar albino rats.
Material and methods: : Total six groups of six animals each were used for the present study. Group I received normal saline 0.9% for 30 days, group II received saline for 30 days and a single dose of DXN (15mg/kg i.p.) on 28th day, group III received standard vitamin E (100 mg/kg p.o.) for 30 days and a single dose of DXN (15 mg/kg i.p.) on 28th day, groups IV-VI received extract (100, 200 & 300 mg/kg p.o., respectively) for 30 days and a single dose of DXN (15 mg/kg i.p.) on 28th day.
Results:
Administration of DXN produced marked biochemical alterations of oxidative stress including increased levels of various biochemical parameters like lactate dehydrogenase (LDH), troponin, serum glutamic-pyruvic transaminase (SGPT), serum glutamic oxaloacetic transaminase (SGOT), alkaline phosphatase (ALP), serum creatinine, blood urea nitrogen (BUN), malonal dialdehyde (MDA) and decreased levels of total protein, antioxidant enzymes- superoxide dismutases (SOD), catalase, glutathione (GSH). But prior administration of hydroalcoholic extract of Vitis vinifera ahead of DXN challenge ameliorated all these biochemical markers.
Conclusion:
The results of the present study revealed the protective effect of hydroalcoholic extract of Vitis vinifera in DXN-induced oxidative stress in heart, liver and kidney.