Proconvulsant effects of estriol-a female ovarian hormone on kianic acid induced seizures in mice

Aakifa Ahmad1*, Md. Naushad Alam2, Abul Kalam Najmi3, Farhan Jalees Ahmad4.
1Department of Pharmacology College of Pharmacy, Monad University, Hapur, Utter Pradesh, INDIA.
2College of Pharmacy, King Khalid University, Abha, KSA.
3Department of Pharmacology, Faculty of Pharmacy, Jamia Hamdard, New Delhi, INDIA.
4Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, INDIA.

ORIGINAL RESEARCH ARTICLE
Volume 2017, Issue 2017, Article ID 134, Page 01-07.

*Author for correspondence
E-mail: akfin.ahmad@gmail.com

ABSTRACT
Background: The estriol which was evaluated for its effect on Kianic acid kindling model of epileptogenesis in mice followed by evaluation on kindling-induced changes in cognitive and motor functions.
Material and methods: Kindling was induced on every alternate day for duration of 45 days and treatment with kianic acid was given at dose ranging from 15 to 30 mg/kg body weight intraperitoneal (i.p) and estriol was also administered at dosage of 0.005 and 0.1 mg/kg through i.p. After induction of kindling the seizure severity was recorded and further percentage incidence of animals kindled at the end of 45 days was also recorded. Spatial learning and cognitive alterations was assessed by Morris water test (MWT) and spontaneous alternation behavior (SAB) while motor function was assessed by grip strength meter.
Results: Estriol increased the rate of kindling in both the sexes of mice at great scale. Percentage incidence of seizures was also intensified. A noticeable decline in the grip strength, spontaneous alteration behavior and Morris water was observed following KA-kindling in pre-treated estriol groups of mice both the sexes.
Conclusion: Control animals developed a seizure score of 4 after the end of 5 weeks, mice treated with estriol exhibited kindling in first two weeks only Clomiphene at dose of 0.9 mg/kg i.p. exhibited anticonvulsant effects. The study displayed that estriol has powerful anticonvulsant effect.
Keywords: Estriol, kainic acid, seizures, clomiphene, spontaneous alteration behavior, grip strength meter.