To investigate the phytochemical constituents present in the Glycyrrhiza glabra stolon and root and estimation of the total phenolic content in ethanolic and aqueous extract. The amount of total phenols was analyzed using a spectrophotometric technique, based on Folin Ciocalteau reagent using gallic acid as standard. The standard curve equation was y=0.007x+0.186 and R2=0.992. The presence of alkaloids, carbohydrates, flavonoids, glycosides, tannins, proteins, phenols, sterols, and resins was revealed by the qualitative examination of Glycyrrhiza glabra stolon and root. The phenolic content in alcoholic and aqueous extract was found to be 244.85 and 232.0 mg/g gallic acid equivalent (GAE) respectively.
Keywords: Phytochemical screening, Total phenolic content, Spectrophotometer, Glycyrrhiza glabra, Gallic acid.