Hepatoprotective effect of darhald (Berberis aristata DC.) against chemically induced hepatic damage

Aisha Siddiqui1*, Anwar Jamal2, Tajuddin3, KMY Amin3.

1Dept. of Ilmul Advia F/o Unani Medicine, Jamia Hamdard, New Delhi-62, India.
2CCRUM, (Presently Department of AYUSH), AYUSH Bhawan, B-Block, INA Market, New Delhi-110023, India.
3Dept. of Ilmul Advia, F/o Unani Medicine, AMU, Aligarh-202002, UP, India.

ORIGINAL RESEARCH ARTICLE
Volume 2017, Issue 2017, Article ID 100, Page 1-5.

ABSTRACT
Background: Berberis aristata is a medicinally important herb and used for centuries in Unani system of medicine for the treatment of various ailments. In the present paper, aqeous-ethanolic extract (50%, v/v) of the bark of Berberis aristata is shown to possess hepatoprotective activity.
Materials and Methods: Pretreatment of rats with the doses of 150 mg/kg and 200 mg/kg, p.o. of the plant extract for seven days significantly ameliorated the liver damage in rats exposed to the hepatotoxic compound galactosamine. The degree of protection was measured by using biochemical parameters like serum glutamate pyruvate transaminases (SGOT), serum glutamate pyruvate transaminases (SGPT), alkaline phosphatase (ALP), serum bilirubin (TB) and metabolic parameters like total protein (TP), serum cholesterol, serum urea and wet liver weight. Since, hepatoprotective effect may be exerted, at least partly due to anti-oxidant activity; therefore, the test drug was also studied for TBARS test (Thiobarbituric Acid Reactive Substance).
Results: Only higher dose of Darhald (200 mg / kg; p.o.) exert significant protection, whereas lower dose (150mg/kg; p.o) was ineffective in reducing SGPT and SGOT concentration significantly against acute damage produced by Galactosamine.
Conclusion: The aqueous-ethanolic extract (50%, v/v) showed a significant hepatoprotective activity comparable with that of Silymarin (100 mg/kg,p.o.). Histopathological studies further confirmed the hepatoprotective activity when compared with the CCl4 treated group.
Keywords: Berberis aristata, Liver, Chemical, SGOT, SGPT, ALP.