Sohail Hussain1,2.
1Department of Biochemistry, Faculty of Medicine, J. N. Medical College, Aligarh Muslim University, Aligarh, INDIA.
2Department of Biochemistry, College of Pharmacy, Jazan University, Jazan, Kingdom of Saudi Arabia (KSA).
ORIGINAL RESEARCH ARTICLE
Volume 2017, Issue 2017, Article ID 116, Page 01-09.
*Author for correspondence
E-mail: [email protected]
ABSTRACT
Aim: To elucidate the antiproliferative effects of Epigallocatechin-3-gallate (EGCG), a green tea polyphenol, in the HPV 18 positive human cervical cancer HeLa cell line.
Material and methods: The viability of the cells was assessed by MTT and Trypan blue dye exclusion method. Cell cycle analysis and apoptosis by FITC-Annexin V were measured by flow cytometry. Protein expression was detected by Western blotting. Morphological changes were observed by phase contrast microscope.
Results: EGCG strongly decreased HeLa cell line proliferation in a dose- and time-dependent manner. Microscopic examination and flow cytometry assay showed that EGCG causes a reduction in cell number by its antiproliferative property. Furthermore, Western blot assay of EGCG treated HeLa cell lines showed activation of caspase-3, caspase-9, cleavage of PARP and decreased Bcl-2 expression.
Conclusion: EGCG strongly inhibited the growth of HPV-positive cervical cancer cells and induce apoptosis.
Aim: To elucidate the antiproliferative effects of Epigallocatechin-3-gallate (EGCG), a green tea polyphenol, in the HPV 18 positive human cervical cancer HeLa cell line.
Material and methods: The viability of the cells was assessed by MTT and Trypan blue dye exclusion method. Cell cycle analysis and apoptosis by FITC-Annexin V were measured by flow cytometry. Protein expression was detected by Western blotting. Morphological changes were observed by phase contrast microscope.
Results: EGCG strongly decreased HeLa cell line proliferation in a dose- and time-dependent manner. Microscopic examination and flow cytometry assay showed that EGCG causes a reduction in cell number by its antiproliferative property. Furthermore, Western blot assay of EGCG treated HeLa cell lines showed activation of caspase-3, caspase-9, cleavage of PARP and decreased Bcl-2 expression.
Conclusion: EGCG strongly inhibited the growth of HPV-positive cervical cancer cells and induce apoptosis.
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