Shahroz Khan1*, G. Jeyabalan2, Ameeduzzafar3, Nassiruddin Ahmad Farooqui 3, Zulfequar Ali4, Mohd Fazil4
1Department of Pharmaceutics, SunRise University, Alwar (Rajasthan), India.
2Alwar Pharmacy College, Alwar Rajasthan, India.
3IIMT College of Medical Science, Merrut, UP, India.
4Jamia Hamdard, New Delhi, India.
ORIGINAL RESEARCH ARTICLE
Volume 4, Issue 1, Page 7-14, January-April 2016.
Article history
Received: 20 March 2016
Revised: 02 April 2016
Accepted: 13 April 2016
Early view: 15 April 2016
*Author for correspondence
E-mail: [email protected]
ABSTRACT
Objective: For the quantitation of Besifloxacin Hydrochloride (BSF), a rapid, linear, precise, accurate and robust method using ultraviolet (UV) spectrophotometer in bulk drug and finished formulation (market formulation and developed in situ gel) was developed.
Material and methods: Shimadzu 1700, Kyoto, Japan, double beam UV spectrophotometer, wavelength accuracy ±0.5 nm and a pair of 1.0 cm matched quartz cells were used to measure absorbance of resulting solution. Reference standard and bulk of BSF was purchased from Avanscure Life Sciences Private Limited, Gurgaon, Haryana. Besifloxacin ophthalmic suspension 0.6% w/v (Besix® eye drops), Ajanta Pharma, containing 5 ml of product was procured from a local pharmacy.
Results: The method was found to be linear for Besifloxacin hydrochloride in the range of 4-12 μg/ml with good correlation coefficient (r2) 0.997), precise with 0.59% RSD, accurate with 98.38-98.74% recovery and robust with 0.54% RSD. The limit of detection and limit of quantitation was found to be 0.589 μg/ml and 1.767 μg/ml respectively.
Conclusion: Proposed method was found to be linear, accurate, precise, robust and suitable for routine quality control analysis for estimation of Besifloxacin hydrochloride in bulk drug and pharmaceutical dosage forms (market formulation and developed in situ gel
Keywords: UV spectrophotometer, Besifloxacin Hydrochloride, Simulated Tear Fluid (STF).
Objective: For the quantitation of Besifloxacin Hydrochloride (BSF), a rapid, linear, precise, accurate and robust method using ultraviolet (UV) spectrophotometer in bulk drug and finished formulation (market formulation and developed in situ gel) was developed.
Material and methods: Shimadzu 1700, Kyoto, Japan, double beam UV spectrophotometer, wavelength accuracy ±0.5 nm and a pair of 1.0 cm matched quartz cells were used to measure absorbance of resulting solution. Reference standard and bulk of BSF was purchased from Avanscure Life Sciences Private Limited, Gurgaon, Haryana. Besifloxacin ophthalmic suspension 0.6% w/v (Besix® eye drops), Ajanta Pharma, containing 5 ml of product was procured from a local pharmacy.
Results: The method was found to be linear for Besifloxacin hydrochloride in the range of 4-12 μg/ml with good correlation coefficient (r2) 0.997), precise with 0.59% RSD, accurate with 98.38-98.74% recovery and robust with 0.54% RSD. The limit of detection and limit of quantitation was found to be 0.589 μg/ml and 1.767 μg/ml respectively.
Conclusion: Proposed method was found to be linear, accurate, precise, robust and suitable for routine quality control analysis for estimation of Besifloxacin hydrochloride in bulk drug and pharmaceutical dosage forms (market formulation and developed in situ gel
Keywords: UV spectrophotometer, Besifloxacin Hydrochloride, Simulated Tear Fluid (STF).
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