Comparative efficacy of the curcumin against H2O2 induced ROS in cervical cancer biopsies and hela cell line

Sohail Hussain1,2
1Department of Biochemistry, Faculty of Medicine, J. N. Medical College, Aligarh Muslim University, Aligarh, INDIA.
2Department of Biochemistry, College of Pharmacy, Jazan University, Jazan, Kingdom of Saudi Arabia (KSA).

ORIGINAL RESEARCH ARTICLE
Volume 2017, Issue 2017, Article ID 123, Page 01-05.

*Author for correspondence
E-mail: shussainamu@gmail.com

ABSTRACT
Background: Antioxidants have played an important role in maintaining physiological homeostasis. Recent literature emphasizes potential therapeutic effects of curcumin (antioxidants) found in natural products that displayed anti-inflammatory and antioxidant effects applicable in preventing oxidative stress induced injury, which characterizes their pathogenesis.
Materials and methods: HeLa cell line and cervical cancer biopsies (CCB) were treated with varying dose of curcumin to determine its effects. Thereafter hydrogen peroxide (0-10nM) – a ROS generating compound was co-cultured with varying doses of curcumin. The effect of these compounds on Superoxide dismutase and Glutathione peroxidase activity was assessed.
Results: The activity of SOD and GPx was found to be affected by the nearly similar magnitude in cervical cancer biopsies & hela cell line.
Conclusion: Comparative efficacies of natural antioxidants were found to be similar activity in cervical cancer biopsies (CCB) and heLa cell line.
Keywords: Curcumin, SOD, GPx, HeLa and Cervical Cancer.