The present global scenario in context to agriculture productivity and loss explores the series of unpleasant surprising. The main objective the present study is to investigate the activity of amylase inhibitor’s (AI’s) from plant storage material i.e. seed, against amylase enzyme of insects Tribolium castaneum and Helicoverpa armigera. Amylase inhibitors was extracted from seeds of Cicer areitinum, Triticum aestivum, Pennisetum typhoides and Zea mays and tested against amylase of two larvae T. castaneun and H. armigera. The protein Quantitation of crude alpha amylase inhibitors was carried out and compared with Bovine serum albumin. The plant seed extracts showed inhibitory activity against amylase enzyme obtained from T. castaneum and H. armigera respectively. The maize amylase inhibitor (MAI’s) extract showed maximum inhibitory activity as compared to other source against amylase isolated from both organisms. The amylase inhibitor extracted from Z. mays showed 82.1 % and 85.12% inhibition against T. castaneum and H. armigera respectively. In the present study all four experimental seed samples posses’ potent insect amylase inhibitors. These inhibitors block the activity of insect’s gut amylase with varying percentage. Which is beneficial to control the pest in agricultural to avoid loss of agricultural yield.