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Umarani V1, Sudhakar M 1, Ramesh A2, Lakshmi BVS1*, Mahipal K1.

1Department of Pharmacology, Malla Reddy College of Pharmacy, Dhulapally, Maisammaguda, Secunderabad, TS, India.
2Department of Pharmacology, Vishnu Institute of Pharmaceutical Education and Research, Narsapur, Hyderabad, TS, India.

ORIGINAL RESEARCH ARTICLE
Volume 3, Issue 3, Page 116-121, September-December 2015.

Article history
Received: 10 September 2015
Revised: 20 September 2015
Accepted: 30 September 2015
Early view: 05 October 2015

*Author for correspondence
E-mail: adithya.neha@gmail.com

ABSTRACT
Background: In present study, protective effect of Rutin against sodium fluoride (NaF)-induced nephrotoxicity was evaluated in rats.
Materials and methods: Renal injury was induced by daily administration of sodium fluoride 600 ppm in distilled water. Group I-control, group II-NaF given through drinking water, group III-vitamin E (100 mg/kg), group IV-rutin (50 mg/kg) and group V (70 mg/kg) along with sodium fluoride once a day for 2 weeks. Protective effects of rutin on sodium fluoride-induced nephrotoxicity in rats were determined for biochemical parameters in urine, serum, various antioxidant enzymes.
Results: Administration of nephrotoxic NaF treatment significantly (P<0.01) increased the level of specific gravity, urea, uric acid, protein, and creatinine clearance level whereas decreased (P<0.01) the pH and creatinine as compared to the control group. Treatment of rutin attenuated the NaF intoxication, dose dependently, and decreased (P<0.01) the specific gravity, urea, uric acid, protein and creatinine clearance while increased the pH and creatinine of urine. Activity of antioxidants catalase, superoxide dismutase, reduced glutathione contents were decreased (P<0.01) whereas lipid peroxidation product was increased. A significant decrease in body and organ weights occurred compared to control group. Treatment with rutin effectively ameliorated the alterations in the studied parameters of rats.
Conclusions: These results suggest that rutin works as an antioxidant In vivo by scavenging reactive oxygen species and this serves to prevent oxidative renal and haematological damage in rat treated with sodium fluoride.
Key words: Sodium fluoride; Rutin; nephrotoxicity; oxidative stress.