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Chandraju S1*, Venkatesh R1, ChidanKumar CS2
1Department of Studies in Sugar Technology, Sir M Vishweshwaraya Post-graduate Center, University of Mysore, Tubinakere, Mandya-571 402, Karnataka, India.
2Department of Engineering Chemistry, Alva’s Institute of Engineering & Technology, Shobhavana Campus, Mijar, Moodbidri, South Canara District-574225, Karnataka, India.

Volume 2, Issue 2, Page 101-104, May-August 2014.

Article history
Received: 20 July 2014
Revised: 2 August 2014
Accepted: 20 August 2014
Early view: 22 August 2014

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The mixture of alkaline copper tartarate and potassium ferrocyanide was found to be useful in the spectrophotometric determination of reducing sugar from papaya peel by hydrolyzing the polysaccharide by varying the concentration of acid, temperature and time of exposure. The absorbance formed at 670 nm was proportional to the concentration of copper; with reference to Cu-glucose equivalence table quantitative estimation of reducing sugar is arrived. The novelty in this method is utilization of Cu (II) oxidative state instead of reduced Cu (I) for analysis which makes it different from usual standard methods. This method is rapid, convenient with a minimal relative percentage error i.e. 0.8%.