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Vishal N Shinde1, Sandip S Nandre1, Zafar S. Khan2*
1Department of Botany, ADMSP’s Late Annasaheb R D Deore, Arts & Science College, Mhasdi, Tal. Sakri, Dist. Dhule- 424304, MS, India.
2Department of Botany, SGVSSP’s Arts, Commerce and Science College Onde, Tal. Vikramgad, Dist. Thane-401605, MS, India.

 

SHORT COMMUNICATION
Volume 2, Issue 1, Jan-April 2014.

Article history
Received: 15 March 2014
Revised: 20 March 2014
Accepted: 20 April 2014
Early view: 28 April 2014

*Author for correspondence
E-mail: vishalshinde1001@gmail.com
Mobile/ Tel.: 0000000000
Keywords:
Methyl benzimidazole-2-yl Carbamate
MIC
Mycotoxicity
Leafy vegetables
Percent Diseases Reduction (PDR).

ABSTRACTIndia has achieved self sufficiency and good degree of stability of vegetable crop production. Leafy vegetables are most essential component of our diet which nourishes with nutrients, minerals and vitamins. The aim of the present work is to evaluate methyl benzimidazole-2-yl carbamate against 10 pathogenic fungi of leafy vegetables in-vitro and in-vivo. The Poisoned food technique was applied for in-vitro fungicides assessment and percent inhibition of mycelia growth over control was calculated. For in-vivo study each leafy vegetable sown in 12×24 M plots in the field. After 10 day of interval, 200 ml spore suspension of each targeted plant pathogenic fungi was mixed in the soil of the respectively field. After 7 days, the diseases symptoms were developed on the leaves of vegetables. Afterwards Minimum Inhibitory Concentration (MIC) in μg/ml of Carbendazim from in vitro results was selected for in- vivo study. The defined concentrations of Methyl benzimidazole-2-yl carbamate was sprayed directly onto the infected leafy vegetables. Efficacy of each fungicide was evaluated by calculating the Percent Diseases Incidence (PDI) and Percent Diseases Reduction (PDR) over control. In-vitro Carbendazim revealed 50.99% inhibition of mycelia growth against all of targeted fungal pathogens. MIC of Carbendazim to all fungal pathogens varied from 40-12000 µg/ml. In vivo studies, Carbendazim was highly effective in controlling disease incidence. The percent disease reduction (PDR) with Carbendazim treatment was maximum 84.64% in all treated leafy vegetables. The use of Carbendazim is very effective against pathogenic fungi of C. esculanta, S. oleracia and T. foenumgraecum in the field condition as they showed complete percent disease reduction (PDR).