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Sudhakar Chaurasiya1*, Narendra Pratap Singh Sengar1, PD Mehta1, J Banweer, Swapna Sahu2.
1Department of Pharmaceutical Chemistry, Sagar Institute of Research & Technology-Pharmacy, SAGE University, Bhopal, M.P., India.
2Faculty of Pharmacy, Nirmala Devi Pharmacy College, Gaurabadshahpur, Jaunpur, U.P., India.

Volume 10, Issue 3, Page 94-100.

*Author for correspondence

Aim: The purpose of this study was to create a new approach for separating the two main flavonoids, quercetin and kaempferol, from Camellia Sinensis leave using the RP-HPLC method.
Material and Methods: The preparative column was used to isolate the flavonoids after the analytical column had first separated and identified them. A third-party, industry-standard analytical RP-HPLC technique was used to determine the presence of quercetin and kaempferol. The software was used to set up the fraction collection, and the chromatogram’s peak times for quercetin (7.42 min) and kaempferol (11.39 min) were recorded.
Results: For quercetin and kaempferol, respectively, the UV area% purity of the obtained components was 86.67 and 89.23. The maximum emission wavelengths of isolated quercetin and conventional quercetin were 256 nm and 365 nm, respectively. The maximum and minimum wavelengths of isolated kaempferol were 259 nm and 361 nm, respectively, as opposed to 267 nm and 368 nm for conventional kaempferol. The FT-IR spectra of quercetin and kaempferol (both standard and isolated) overlap. Indicating -OH groups, the major spectral peaks were seen at >3300 cm-1 and 1600–1700 cm-1 (representing the carbonyl function).
Conclusion: It has been discovered that the technique is exact, accurate, linear, durable, cost-effective, sensitive, and capable of quickly isolating both of the essential phytoconstituents from the extracted natural materials. The study thus opens up possibilities for using this technology in industry-based activities and quality control procedures at various operability scales.
Keywords: Quercetin, Kaempferol, Camellia sinensis, RP-HPLC, Flavonoid, Phenolic.