ABSTRACT
Background: The present study was designed to evaluate the effect of alpha lipoic acid and nebivolol on doxorubicin (DOX)-induced cardiotoxicity and oxidative stress in mice.
Materials and Methods: Mice were pre-treated with alpha lipoic acid (16 mg/kg, i.p.) and nebivolol (1 mg/kg, p.o.) for 7 days and received a single dose of DOX (15 mg/kg, i.p.) on day 8. After 36 h, serum chemistry and relevant enzymes in cardiac tissues were assessed. Histopathology of the heart tissues was performed.
Results: Alpha lipoic acid and Nebivolol produced significant reduction in serum lactate dehydrogenase (LDH), creatine kinase-muscle/brain (CK-MB), serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT) and cardiac lipid peroxides (MDA) levels, and elevation in cardiac antioxidant enzymes [glutathione (GSH), and superoxide dismutase (SOD) and catalase (CAT)] levels. These results were also supported by the histological findings.
Conclusion: Results provide clear evidence that alpha lipoic acid and nebivolol offered significant protection against DOX-induced cardiotoxicity in mice. The cardioprotective effects are attributed to the antioxidant effects of alpha lipoic acid and nebivolol.
Keywords: Doxorubicin, Oxidative Stress, Alpha Lipoic Acid, Nebivolol.