Preclinical evaluation of herbal plant in potassium oxonate-induced hyperuricemia

AR Ziyaurrahman1*, Pooja Kale2, Swati Narkhede2.

1M.C.E. Society’s Allana College of Pharmacy, Azam Campus, Camp, Pune-411001, MS, India.
2Department of Pharmacology, AISSMS College of Pharmacy, Kennedy Road, Pune: 411 001, MS, India.

ORIGINAL RESEARCH ARTICLE
Volume 2017, Issue 2017, Article ID 108, Page 1-5.

ABSTRACT
Objective: The present study was undertaken to investigate the anti-gouty arthritic activity of Adhatoda vasica Nees aqueous leaves extract (AVLE) using potassium oxonate-induced hyperuricemia in mice.
Material and methods: Potassium oxonate (PO) induced hyperuricemia in mice were determined by in vivo experiments. PO causes hyperuricemia in one hour after i.p. administration. Thus mice were administered PO followed by AVLE and standard drug treatment of allopurinol study conducted for 7 days finally measuring the serum levels of uric acid, xanthine oxidase, and xanthine dehydrogenase etc.
Results: The evaluation of anti-gouty arthritic potential by oral administration of AVLE (100–400 mg/kg) evoked a significantly decrease in the serum levels of uric acid, xanthine oxidase (XOD), xanthine dehydrogenase (XDH) in treated mice. Finally, the study concluded that in AVLE the flavonoid constituents impart enzyme inhibitory activity thus reducing levels of xanthine oxidase, xanthine dehydrogenase, and serum uric acid
Conclusions: The results obtained in this study indicate that AVLE possesses potential anti-gouty arthritic activity.
Keywords: XOD-Xanthine oxidase, XDH-Xanthine dehydrogenase, PO-Potassium oxonate.