The mixture of alkaline copper tartarate and potassium ferrocyanide was found to be useful in the spectrophotometric determination of reducing sugar from papaya peel by hydrolyzing the polysaccharide by varying the concentration of acid, temperature and time of exposure. The absorbance formed at 670 nm was proportional to the concentration of copper; with reference to Cu-glucose equivalence table quantitative estimation of reducing sugar is arrived. The novelty in this method is utilization of Cu (II) oxidative state instead of reduced Cu (I) for analysis which makes it different from usual standard methods. This method is rapid, convenient with a minimal relative percentage error i.e. 0.8%.