Shahnaz Sultana1,2, Mohammed Ali1*, Showkat Rassol Mir1
1Phytochemistry Research Laboratory, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi-110062, INDIA.
2College of Pharmacy, Jazan University, Jazan, SAUDI ARABIA.
ORIGINAL RESEARCH ARTICLE
Volume 5, Issue 4, Page 217-224.
*Author for correspondence
E-mail: maliphyto@gmail.com
ABSTRACT
Background: Adenanthera pavonina L. (Leguminosae) is a fast-growing, attractive, perennial tree found in southern China, India, Florida and South America. Our study was planned to isolate chemical constituents from the leaves of A. pavonina and E. variegata and roots of H. eichwaldii and to characterize their structures.
Material and Methods: The air-dried powders of the leaves of A. pavonina and E. Variegata and roots of H. eichwaldii were exhaustively extracted with methanol individually and the concentrated each extract was adsorbed on silica gel (60-120 mesh) separately for the preparation of slurries. Each dried slurry was chromatographed over silica gel column packed in petroleum ether. The columns were eluted with petroleum ether, chloroform, and methanol, successively, in order of increasing polarity to isolate the compounds.
Results: Phytochemical investigation of a methanolic extract of the leaves of A. pavonina afforded 1-tricosanol (1) and α-D-glucopyranosyl-(6→1′)-α-D-glucopyranosyl-(6′→1′′)-α-D-glucopyranosyl-(6′′→1′′′)-α-D-glucopyranoside (α-6-O-D-tetraglucoside, (2) Column chromatography of a methanolic extract of the leaves of E. variegata gave 1-hexatetracontanol, (3) and 1-octanyloxy- O-β-D-glucopyranosyl-(6′→1′′) -O-β-D-glucopyranoside, (4) A methanolic extract of the roots of H. eichwaldii on subjection to silica gel column furnished n-nonanyl oleate, (5) n-dodecanyl stearate, (6) triarachidin, (7) stigmast-5,23–dien-3β –ol (23-epistigmasterol, (8) n-undecanyl n-docosanoate (n-undecanyl behenate, (9) and (17Z)-n-tetracont -17-enoic acid (10). The structures of all the isolated phytoconstituents have been established on the basis of spectral data analysis and chemical reactions.
Conclusion: The leaves of A. pavonina possessed an aliphatic alcohol and a tetraglucoside. A long chain alcohol and an alkyloxy diglucoside were isolated from the E. variegata leaves. The H. eichwaldii roots afforded fatty acid esters, a phytosterol, and a long chain unsaturated fatty acid.
Keywords: Adenanthera pavonina, Erythrina variegata, Heliotropium eichwaldii roots, Chemical constituents, Isolation, Characterization.
Background: Adenanthera pavonina L. (Leguminosae) is a fast-growing, attractive, perennial tree found in southern China, India, Florida and South America. Our study was planned to isolate chemical constituents from the leaves of A. pavonina and E. variegata and roots of H. eichwaldii and to characterize their structures.
Material and Methods: The air-dried powders of the leaves of A. pavonina and E. Variegata and roots of H. eichwaldii were exhaustively extracted with methanol individually and the concentrated each extract was adsorbed on silica gel (60-120 mesh) separately for the preparation of slurries. Each dried slurry was chromatographed over silica gel column packed in petroleum ether. The columns were eluted with petroleum ether, chloroform, and methanol, successively, in order of increasing polarity to isolate the compounds.
Results: Phytochemical investigation of a methanolic extract of the leaves of A. pavonina afforded 1-tricosanol (1) and α-D-glucopyranosyl-(6→1′)-α-D-glucopyranosyl-(6′→1′′)-α-D-glucopyranosyl-(6′′→1′′′)-α-D-glucopyranoside (α-6-O-D-tetraglucoside, (2) Column chromatography of a methanolic extract of the leaves of E. variegata gave 1-hexatetracontanol, (3) and 1-octanyloxy- O-β-D-glucopyranosyl-(6′→1′′) -O-β-D-glucopyranoside, (4) A methanolic extract of the roots of H. eichwaldii on subjection to silica gel column furnished n-nonanyl oleate, (5) n-dodecanyl stearate, (6) triarachidin, (7) stigmast-5,23–dien-3β –ol (23-epistigmasterol, (8) n-undecanyl n-docosanoate (n-undecanyl behenate, (9) and (17Z)-n-tetracont -17-enoic acid (10). The structures of all the isolated phytoconstituents have been established on the basis of spectral data analysis and chemical reactions.
Conclusion: The leaves of A. pavonina possessed an aliphatic alcohol and a tetraglucoside. A long chain alcohol and an alkyloxy diglucoside were isolated from the E. variegata leaves. The H. eichwaldii roots afforded fatty acid esters, a phytosterol, and a long chain unsaturated fatty acid.
Keywords: Adenanthera pavonina, Erythrina variegata, Heliotropium eichwaldii roots, Chemical constituents, Isolation, Characterization.
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