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Md Imaduddin*, Chandrashekhar VM, Firasat Ali, Gudasi KB, Parvinder Singh.
Department of Pharmacology, HSK College of Pharmacy, Bagalkot–587101, Karnataka, India.

ORIGINAL RESEARCH ARTICLE
Volume 2, Issue 3, Page 136-141, September-December 2014.

Article history
Received: 5 November 2014
Revised: 25 November 2014
Accepted: 30 November 2014
Early view: 15 December 2014

*Author for correspondence
E-mail: imadpharma111@gmail.com

ABSTRACT

Background: In the field of medicinal chemistry quinazolinone has gained unique place due to anti-microbial, anti-inflammatory, analgesic, anti-convulsant, anti-hypertensive, diuretic and anti-viral activity. 3-methoxysalicylaldehyde-2-aminobenzoylhydrazone (AQF) was complexed with transition metal nickel (Ni) and used to evaluate anti-arthritic activity in Freund’s complete adjuvant (FCA) induced arthritis in Sprague–Dawley (SD) female rats.
Materials and methods: The anti-arthritic activity of Ni-AQF (5mg/kg and 10mg/kg) was evaluated in rats subjected to Adjuvant Arthritis (AA) by the sub-plantar injection of 0.1ml of FCA. The anti-arthritic activity of Ni-AQF was assessed by determining the Paw oedema volume, vascular permeability test, histamine level in blood. It was also determined by radiographic analysis, Histopathological assessment of arthritis induced joints.
Results: Animals treated with Ni-AQF showed significant reduced paw oedema, release of histamine into the blood, redness, swelling, and erythema at ankle joints. Furthermore Ni-AQF treatment showed reduced infiltration of lymphocytes, swelling deformities and joint spaces. Histopathological assessment of rats treated with Ni-AQF showed mild Proliferation and infiltration of mononuclear cells, mildsub-chondral bone erosion and superficial cartilage damage as compared to control group.
Conclusions: These results suggested the protective role of Ni-AQFin FCA induced chronic arthritis model. In our present study, Diclofenac sodium was used as a reference standard, which is one of the familiar non-Steroidal anti-inflammatory drugs (NSAID’s) for the initial treatment for rheumatoidarthritis (RA). Further, studies are required to elucidate the detail mechanism of action of this agent at molecular level to explore the therapeutic benefits.

Keywords: Rheumatoid arthritis, paw oedema, inflammation, 3-methoxysalicylaldehyde-2-aminobenzoylhydrazone.